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KMID : 1134820120410081079
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 8 p.1079 ~ p.1085
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Effects of Vitexin from Mung Bean on 3T3-L1 Adipocyte Differentiation and Regulation According to Adipocytokine Secretion
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Wi Hae-Ri
Choi Mun-Ji
Choi Se-Lim
Kim Ae-Jung
Lee Myoung-Sook
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Abstract
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Obesity is an important issue worldwide as it may associated with increased prevalence of metabolic diseases. Mung bean is known as a functional food for decreasing the glycemic index and lipid profile of plasma. The purpose of this study was to investigate the anti-obesity effects of vitexin from mung bean on the regulation of adipocyte differentiation and adipocytokine secretion. When 3T3-L1 adipocytes were treated with vitexin from days 0 to 14 at various levels of 25, 50, 100, and 200 μM, there was no change in cell viability. Vitexin treatment at 50, 100, and 200 μM decreased triacylglycerol levels in cells, but only 100 μM vitexin induced lipolysis. At 200 μM of vitexin, phosphorylation of p38 and ERK, which causes secretion of inflammatory adipocytokines, was depressed, whereas there was an increase in expression of PPARγ, the key regulator of adipocyte differentiation. Phosphorylation of AMPK increased at 100 μM vitexin. TNF-α and aP2 mRNA expression increased at 25 μM vitexin, whereas only TNF-α mRNA expression increased at 200 μM vitexin. Further, the mRNA levels of TNF-α and aP2 decreased at other concentrations in a dose-dependent manner. Since we observed that mRNA expression of C/EBP, SREBP1, and PPARγ did not change upon vitexin treatment, our future studies will investigate other genes such as mTOR, which is related with apoptosis signaling, or SIRT1, which is associated with inhibition of adipogenesis. Our results indicate that vitexin at concentrations between 100 and 200 μM is suitable in vivo for the development of mung bean as an anti-obesity therapy or functional food.
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KEYWORD
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mung bean, vitexin, adipocytokines, PPARγ, TNF-α
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